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- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
(3-Bromo-4-methylphenyl)methanol
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- 供应商:
无锡云萃生物
- CAS号:
68120-35-4
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验Yeast IF with Methanol/Acetone Dehydration
Yeast IF with Methanol/Acetone Dehydration David Amberg Note this protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual. This is the protocol to use with the Botstein anti-actin
Methanol Fixation for Immunofluorescence
Methanol fixation works by precipitating proteins, and as such it is a quick method (2-5)minutes is enough time for most antibodies/proteins). Diffuse proteins may be lost,however, in which case PFA fixation may be a better choice
Yeast IF with Methanol/Acetone Dehydration
This is the protocol to use with the Botstein anti-actin rabbit antibodies. Grow cells at the appropriate temperature to 5x10E6 in 5 mls YPD. Add 0.5mls 37 formaldehyde (best grade) and incubate on the roller at same temp. for 10






