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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
MMP-3 Green™ substrate
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
1 mg
关键词：MMP-3 Green 基质金属蛋白酶绿色荧光底物详细如下
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix. These enzymes are responsible for the breakdown of connective tissues and are important in bone remodeling, the menstrual cycle and repair of tissue damage. While the exact contribution of MMPs to certain pathological processes is difficult to assess, MMPs appear to play a key role in the development of arthritis as well as in the invasion and metastasis of cancer. MMPs tend to have multiple substrates, with most family members having the ability to degrade different types of collagen along with elastin, gelatin and fibronectin. It is quite difficult to find a substrate that is selective to a single MMP enzyme. This FRET substrate is designed to have a relative high selectivity to MMP-3. It is used to monitor the MMP-3 activity. It can also be used to screening MMP-3 inhibitors when a purified MMP-3 enzyme is used. This FRET substrate is based on our TF2/TQ2 FRET pair. Upon MMP-3 hydrolysis the fluorescence of MMP-3 Green™ FRET peptide substrate is increased since the TF2/TQ2 FRET pair is separated. The fluorescence increase is proportional to the MMP-3 enzyme activities.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-100510I0 | iFluor™ 680 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510I1 | iFluor™ 680 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510J0 | iFluor™ 700 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510J1 | iFluor™ 700 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510K0 | iFluor™ 710 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510K1 | iFluor™ 710 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510L0 | iFluor™ 750 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510L1 | iFluor™ 750 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510M0 | iFluor™ 790 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510M1 | iFluor™ 790 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510N0 | iFluor™ 800 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510N1 | iFluor™ 800 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510O0 | iFluor™ 810 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510O1 | iFluor™ 810 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510P0 | iFluor™ 820 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510P1 | iFluor™ 820 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510Q0 | iFluor™ 840 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510Q1 | iFluor™ 840 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510R0 | iFluor™ 860 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510R1 | iFluor™ 860 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510S0 | iFluor™ A7 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510S1 | iFluor™ A7 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510T0 | mFluor™ Blue 570 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510T1 | mFluor™ Blue 570 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510U0 | mFluor™ Green 620 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510U1 | mFluor™ Green 620 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510V0 | mFluor™ Red 700 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510V1 | mFluor™ Red 700 Anti-human CD5 Antibody *L17F12* | 500tests |
| AAT-100510W0 | mFluor™ Red 780 Anti-human CD5 Antibody *L17F12* | 100tests |
| AAT-100510W1 | mFluor™ Red 780 Anti-human CD5 Antibody *L17F12* | 500tests |
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文献和实验人基质金属蛋白酶-3（MMP-3）酶联免疫分析（ELISA）
被单抗的微孔中依次加入 人基质金属蛋白酶 -3 （ MMP-3 ） ，再与 HRP 标记的羊抗人抗体结合，形成抗体 - 抗原 - 酶标抗体复合物，经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 人基质金属蛋白酶 -3 （ MMP-3 ） 呈正相关。用酶标仪在 450nm 波长下测定吸光度（ OD 值），通过标准曲线计算样品中 人基质金属蛋白酶 -3 （ MMP-3 ） 浓度。 试剂盒组成
人基质金属蛋白酶-9（MMP-9）酶联免疫分析（ELISA）
依次加入 人基质金属蛋白酶 9 （ MMP-9 ） ，再与 HRP 标记的 基质金属蛋白酶 9 （ MMP-9 ） 抗体结合，形成抗体 - 抗原 - 酶标抗体复合物，经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 人基质金属蛋白酶 9 （ MMP-9 ） 呈正相关。用酶标仪在 450nm 波长下测定吸光度（ OD 值），通过标准曲线计算样品中 人基质金属蛋白酶 -9 （ MMP-9 ） 浓度。 试剂盒组成
被单抗的微孔中依次加入基质金属蛋白酶 13 （ MMP-13 ) ， 再与 HRP 标记的 MMP-13 抗体结合，形成抗体 - 抗原 - 酶标抗体复合物，经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的基质金属蛋白酶 13 （ MMP-13 ) 呈正相关。用酶标仪在 450nm 波长下测定吸光度（ OD 值），通过标准曲线计算样品中人基质金属蛋白酶 13 （ MMP-13 ) 浓度。 试剂
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