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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
2-((4-Bromophenyl)thio)ethanamine
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
69326-62-1
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验GST融合蛋白的准备 Preparation of Glutathione
Buffers, Solutions, and Reagents Reduced glutathione, 20 mM prepared in 50 mM Tris-HCl (pH 8.0) Isopropyl-β-D-thio-galactoside (IPTG) LB broth, containing appropriate antibiotic selection Lysis buffer: PBS + 1% Triton X-100, supplemented
ALPHA FACTOR ARREST AND RELEASE
Alpha Factor Arrest and Release Alpha factor stock is 10-3 M in 0.1N HCl (i.e. resuspend 5 mg alpha factor in 2.97 ml 0.1N HCl). To arrest BAR1 strains, the pheromone should be diluted to a final concentration
centrifugation in density gradients ofPercoll. Arch. Biochem. Biophys. 217, 312-323. 5. Leaver, C. J., Hack, E., and Forde, B. G. (1983) Protein synthesis by isolated plantmitochondria. Methods Enzymol. 97,476-484. 6. Day, D. A., Neuburger, M










