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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
Aminopropargyl dUTP [5-Propargylamino-2'-deoxyuridine-5'-triphosphate]*CAS：1380329-75-8*
- CAS号：
1380329-75-8
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
10 umoles
齐源生物专业代理美国AAT Bioquest产品，并有大量储存现货欢迎致电
关键词：脱氧尿苷5'-三磷酸酯 Aminopropargyl dUTP*CAS：1380329-75-8*详细如下
The amine-modified deoxyuridine 5'-triphosphates (such as 5-propargylamino-2'-deoxyuridine-5'-triphosphate) can be used to produce amine-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. Aminopropargyl dUTP can be readily incorporated into DNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminopropargyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled nucleotides that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
关键词：脱氧尿苷5'-三磷酸酯 Aminopropargyl dUTP*CAS：1380329-75-8*详细如下
The amine-modified deoxyuridine 5'-triphosphates (such as 5-propargylamino-2'-deoxyuridine-5'-triphosphate) can be used to produce amine-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. Aminopropargyl dUTP can be readily incorporated into DNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminopropargyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled nucleotides that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-10455150 | AF488 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-10455151 | AF488 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-10455160 | AF555 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-10455161 | AF555 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-10455170 | AF594 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-10455171 | AF594 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-10455180 | AF647 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-10455181 | AF647 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-10455190 | AF680 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-10455191 | AF680 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104551A0 | AF700 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104551A1 | AF700 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104551B0 | AF750 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104551B1 | AF750 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104551B2 | AF750 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104551C0 | APC Anti-human CD45 Antibody *HI100* | 25tests |
| AAT-104551C1 | APC Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104551C2 | APC Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104551D0 | APC/Cy7 Anti-human CD45 Antibody *HI100* | 25tests |
| AAT-104551D1 | APC/Cy7 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104551D2 | APC/Cy7 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104551E0 | APC/AF750 Anti-human CD45 Antibody *HI100* | 25tests |
| AAT-104551E1 | APC/AF750 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104551E2 | APC/AF750 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104551F0 | APC/iFluor™ 700 Anti-human CD45 Antibody *HI100* | 25tests |
| AAT-104551F1 | APC/iFluor™ 700 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104551F2 | APC/iFluor™ 700 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104551G0 | APC/iFluor™ 750 Anti-human CD45 Antibody *HI100* | 25tests |
| AAT-104551G1 | APC/iFluor™ 750 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104551G2 | APC/iFluor™ 750 Anti-human CD45 Antibody *HI100* | 500tests |
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脱氧尿苷5'-三磷酸酯 Aminopropargyl dUTP*CAS：1380329-75-8*
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