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文献和实验There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
Generation of Dendritic Cells from Rat Bone Marrow
could not be provided in vitro (3 ,4 ). The availability of recombinant cytokines and the observations that DC in culture are dependent on cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF) (5 ,6 ) and IL-4 (7 ,8 ) have revolutionized DC
The first transgenic experiment was carried out more than 15 yr ago, and transgenesis is now a routine technique for the study of gene and cell function, and the development of animal models of disease. The mouse remains the species of choice
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