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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
Benzalkonium chloride
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
63449-41-2
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验Competent cells, calcium chloride method, E. coli, description
, CaCl2 method, E. coli, long protocol Keywords: competent cells; E. coli; calcium chloride; Zappe CaCl2 competent cells Description Short protocol Related Contents Contributor: : Zappe, H
in cesium chloride-ethidium bromide gradients (1). Briefly, cells containing the desired plasmid or cosmid are harvested by centrifugation, incubated in a lysozyme buffer, and treated with alkaline detergent. Detergent solubilized proteins and membranes
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS






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