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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 英文名:
Human Transferrin protein
- 规格:
10 μg/50 μg/100 μg/1 mg
| 规格: | 10 μg | 产品价格: | ¥588.0 |
|---|---|---|---|
| 规格: | 50 μg | 产品价格: | ¥1980.0 |
| 规格: | 100 μg | 产品价格: | ¥3416.0 |
| 规格: | 1 mg | 产品价格: | 询价 |
重组人转铁蛋白 (TF) - PRP2041
本品为高纯度重组人转铁蛋白,采用人胚胎肾细胞(HEK293)表达系统制备,适用于细胞培养及相关体外研究应用。产品以冻干粉形式提供,具有高纯度、低内毒素等特点,可满足科研实验中对转铁蛋白的精细需求。
产品特性
- 表达宿主:人胚胎肾细胞(HEK293),确保蛋白修饰接近天然人源蛋白
- 蛋白长度:全长690个氨基酸,预测分子量76.6 kDa
- 纯度:经SDS-PAGE检测,纯度≥95%
- 内毒素水平:每1 μg蛋白质中EU含量<1.0(LAL法检测)
- 标签:无标签,C末端带有聚组氨酸标签(实验设计中便于检测与纯化)
应用建议
本品适用于多种细胞培养体系,尤其是对血清成分敏感或无血清培养环境。推荐复溶浓度不低于100 µg/mL,并可根据具体实验体系进一步稀释。请注意,复溶后的产品建议分装保存,并避免反复冻融。
保存与运输
- 保存条件:冻干粉应在-20℃以下干燥环境中保存;复溶后可在4℃稳定保存2-7天,长期保存需置于-20℃并建议添加0.1% HSA或BSA作为稳定剂
- 运输条件:常温运输
背景信息
转铁蛋白(Transferrin, Tf)是一种铁离子转运蛋白,主要在肝脏中合成,也可在大脑中由少突胶质细胞和脉络丛合成。其在脑脊液(CSF)中存在的两种亚型——脑型Tf和血清型Tf,在糖基化结构上具有显著差异,可能与CSF的生产调节有关,近年来被认为是有潜力的生物标志物。
产品规格与价格
| 规格 | 价格(元) | 货期 |
|---|---|---|
| 10 μg | 588 | 现货,次日发货 |
| 50 μg | 1980 | 现货,次日发货 |
| 100 μg | 3416 | 现货,次日发货 |
| 1 mg | 请咨询 | 请咨询 |
注意:本品仅供科研使用,不适用于人类或临床诊断。使用前请仔细阅读注意事项,并遵循相关实验操作规范。
更多详细信息可参考UniProt数据库:P02787
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Recombinant Human Transferrin (TF) - PRP2041
This product is a high‑purity recombinant human transferrin produced in a human embryonic kidney (HEK293) expression system, suitable for cell‑culture and related in‑vitro research applications. Supplied as a lyophilized powder, it features high purity and low endotoxin levels, meeting the stringent requirements for transferrin in scientific experiments.
Product Characteristics
- Expression host: Human embryonic kidney cells (HEK293), ensuring post‑translational modifications closely resemble native human protein
- Protein length: Full‑length 690 amino acids, predicted molecular weight 76.6 kDa
- Purity: ≥95 % as determined by SDS‑PAGE
- Endotoxin level: <1.0 EU per µg protein (LAL assay)
- Tag: Tag‑free; C‑terminal poly‑histidine tag included for detection and purification in experimental designs
Recommended Applications
The product is suitable for a variety of cell‑culture systems, especially those sensitive to serum components or requiring serum‑free conditions. Reconstitute to a concentration of at least 100 µg/mL; further dilutions can be made according to the specific experimental setup. Aliquot the reconstituted solution and avoid repeated freeze‑thaw cycles.
Storage and Shipping
- Storage conditions: Lyophilized powder should be stored dry at ≤ −20 °C; after reconstitution it remains stable at 4 °C for 2–7 days. For long‑term storage keep at −20 °C and consider adding 0.1 % HSA or BSA as a stabilizer
- Shipping conditions: Ambient temperature
Background
Transferrin (Tf) is an iron‑binding transport protein primarily synthesized in the liver, with additional production in the brain by oligodendrocytes and the choroid plexus. The two isoforms present in cerebrospinal fluid (CSF)—brain‑type Tf and serum‑type Tf—exhibit distinct glycosylation patterns, which may be linked to CSF production regulation. Recently, these isoforms have been explored as promising biomarkers.
Product Specifications and Pricing
| Specification | Price (CNY) | Lead Time |
|---|---|---|
| 10 µg | 588 | In stock, next‑day shipment |
| 50 µg | 1 980 | In stock, next‑day shipment |
| 100 µg | 3 416 | In stock, next‑day shipment |
| 1 mg | Contact us | Contact us |
Note: This product is for research use only and is not intended for human or clinical diagnostic applications. Please read all precautionary statements carefully and follow applicable laboratory safety protocols before use.
For additional details, refer to the UniProt database: P02787
Note: Information above may change due to product optimization or upgrades; please contact YaKeYin Co., Ltd. for the most current data.
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文献和实验Methods for Manipulation of Transferrin-Binding Proteins
To obtain iron, a necessary nutrient, meningococci and several other human and veterinary pathogens have iron-acquisition systems, which are expressed in vivo during infection. One target of iron-acquisition systems is transferrin (Tf
Contribution of CE to the Analysis of Protein or Peptide Biomarkers
from human sample, two detailed protocols are given: the analysis from CSF of five amyloid peptide, biomarkers of the Alzheimer disease, and the analysis of sialoforms of transferrin from human serum.
Introduction of human 14-3-3 proteins
The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14
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