IL-8 - Human IL-8 protein

重组人白细胞介素-8 (IL-8) 蛋白，无标签

产品概述

本品为高纯度重组人源白细胞介素-8 (IL-8/CXCL8) 蛋白冻干粉。该蛋白由大肠杆菌表达系统制备，无标签，保留了其天然的生物活性，专为严格的细胞培养与免疫学研究需求而设计。

产品详情

• 产品名称：IL-8 - Human IL-8 protein
• 英文名称：Human IL-8 protein
• 货号：PRP1022
• 蛋白序列：氨基酸序列来源于人源 IL-8 (UniProt: P10145) (Ser28-Ser99)。
• 表达宿主：大肠杆菌
• 种属来源：人
• 分子量：预测分子量为 8 kDa。在还原性SDS-PAGE中表观分子量约为 8 kDa。
• 蛋白长度：由72个氨基酸（N端带甲硫氨酸）组成。
• 纯度：经SDS-PAGE检测，纯度 ≥ 98% 。
• 内毒素水平：采用LAL法检测，每1μg蛋白质中EU含量 < 1.0，适用于对内毒素敏感的细胞实验。
• 产品形式：白色冻干粉末，由无菌的150mM NaCl, 20mM Tris (pH 7.0)缓冲液冻干而成。

生物活性验证

通过ELISA结合实验进行功能性验证：以200 ng/mL (100μL/孔)浓度固定后人IL-8蛋白可特异性结合小鼠抗人IL-8抗体，测得小鼠抗IL-8抗体的EC₅₀为66.4 ng/mL。

使用说明

• 复溶建议：开盖前请先离心。推荐使用产品附带的复溶缓冲液（Reconstitution Buffer）进行复溶，初始浓度建议不低于100 µg/mL，后续可根据具体实验要求进行稀释。
• 保存条件：
  • 冻干粉：请保存于-20℃以下的干燥环境中。
  • 复溶后：复溶后的蛋白液可在4℃下保存2-7天。如需长期保存，请置于-20℃以下，并建议添加载体蛋白（如0.1% HSA或BSA）以防止吸附，分装保存以避免反复冻融。
• 运输条件：常温运输。

背景信息

白细胞介素-8 (IL-8)，又称CXCL8，是一种属于CXC家族的趋化因子，其基因ID为3576。它由多种细胞类型（如单核细胞、巨噬细胞、成纤维细胞、内皮细胞及某些肿瘤细胞）在炎症刺激下分泌产生。IL-8通过与其受体CXCR1和CXCR2结合，在炎症反应中发挥核心作用，主要负责招募中性粒细胞、T细胞和嗜碱性粒细胞至感染或损伤部位。

该蛋白在肿瘤微环境、自身免疫性疾病及感染性疾病的研究模型中具有重要应用价值。

规格与价格

规格	价格 (人民币)	货期
10 μg	588	现货（次日发货）
50 μg	1989	现货（次日发货）
100 μg	3589	现货（次日发货）
1 mg	请咨询	请咨询

警告：本产品仅限于科研使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。对于因使用本产品而可能导致的专利侵权或其他违规行为，我们不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Interleukin-8 (IL-8) Protein, Tag Free

Product Overview

This product is a highly purified recombinant human Interleukin-8 (IL-8/CXCL8) protein lyophilized powder. It is produced in an E. coli expression system, tag-free, retains its native biological activity, and is designed for demanding cell culture and immunology research applications.

Product Details

• Product Name: IL-8 - Human IL-8 protein
• English Name: Human IL-8 protein
• Catalog Number: PRP1022
• Protein Sequence: Amino acid sequence derived from human IL-8 (UniProt: P10145) (Ser28-Ser99).
• Expression Host: Escherichia coli
• Species Source: Human
• Molecular Weight: Predicted molecular weight is 8 kDa. Apparent molecular weight is approximately 8 kDa as determined by reducing SDS-PAGE.
• Protein Length: Consists of 72 amino acids (with an N-terminal Methionine).
• Purity: ≥ 98% as determined by SDS-PAGE.
• Endotoxin Level: < 1.0 EU per 1 μg of protein, determined by LAL assay. Suitable for cell culture experiments sensitive to endotoxins.
• Product Form: White lyophilized powder, lyophilized from a sterile solution of 150 mM NaCl, 20 mM Tris (pH 7.0).

Bioactivity Validation

Functional validation by ELISA binding assay: Immobilized Human IL-8 protein at 200 ng/mL (100μL/well) specifically binds to a mouse anti-human IL-8 antibody, yielding an EC₅₀ of 66.4 ng/mL for the mouse anti-IL-8 antibody.

Usage Instructions

• Reconstitution Recommendation: Centrifuge the vial before opening. It is recommended to reconstitute using the provided Reconstitution Buffer to a stock concentration of no less than 100 µg/mL, which can then be further diluted according to specific experimental requirements.
• Storage Conditions:
  • Lyophilized Powder: Store below -20°C in a dry environment.
  • After Reconstitution: The reconstituted protein solution can be stored at 4°C for 2-7 days. For long-term storage, store below -20°C. It is advisable to add a carrier protein (e.g., 0.1% HSA or BSA) to prevent adsorption and to aliquot to avoid repeated freeze-thaw cycles.
• Shipping Conditions: Shipped at ambient temperature.

Background Information

Interleukin-8 (IL-8), also known as CXCL8, is a chemokine belonging to the CXC family, with Gene ID 3576. It is secreted by various cell types (such as monocytes, macrophages, fibroblasts, endothelial cells, and certain tumor cells) in response to inflammatory stimuli. IL-8 plays a central role in inflammatory responses by binding to its receptors, CXCR1 and CXCR2, primarily recruiting neutrophils, T cells, and basophils to sites of infection or injury.

This protein is valuable in research models investigating the tumor microenvironment, autoimmune diseases, and infectious diseases.

Specifications & Pricing

Specification	Price (RMB)	Availability
10 μg	588	In Stock (Ships Next Day)
50 μg	1989	In Stock (Ships Next Day)
100 μg	3589	In Stock (Ships Next Day)
1 mg	Please Inquire	Please Inquire

Warning: This product is for research use only. Not intended for human or diagnostic use. The user should ensure that its application does not infringe any patents or licensing regulations. We assume no responsibility for any patent infringement or other violations that may arise from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.