Poly(I:C)(50MG)

Ampicillin(50mg/ml)

  Ampicillin (50mg/ml) Ampicillin Dissolve Ampicillin (Na salt from Sigma, A-9518) in H2 0. Store 1 ml aliquots at 20o C in common freezer for stock solutions   上一篇：PREPARATION

POLY-L-LYSINE COATED SLIDES

POLY-L-LYSINE COATED SLIDES SOLUTIONS --5% Chromic acid --0.01% Poly-L-Lysine (PLL) - made from a dry protein, with distilled water. (100mg/1 litre) This procedure can be carried out on either a ski or carousel staining

Purification of poly(A)+ RNA

1 mg of total RNA dissolved in RNase-free water to 300 ul of the Oligotex-dT30 suspension. 2. Incubate for 3 minutes at 65 C. Chill on ice. 3. Add 0.2 volume of 5M NaCl. Incubate for 10 minutes at 37 C. 4. Centrifuge for 3 minutes