- 询价
- AAT Bioquest
- AAT-11001
- 进口
- 2025年07月15日
7 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*30931-67-0
- CAS号：
30931-67-0
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
1 L
齐源生物专业代理美国AAT Bioquest产品，并有大量储存现货欢迎致电
关键词：ReadiUse™ ABTS 底物溶液 *针对 HRP 偶联物的 ELISA 检测进行了优化*30931-67-0详细如下：
Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product. This chromogenic reaction is widely used for quantify HRP in ELISA assays. The oxidized ABTS product has the absorption maximum of 420 nm that can easily be followed with a spectrophotometer. ReadiUse™ ABTS Solution is optimized for ELISA assays that use HRP or HRP-labeled conjugates and hydrogen peroxide in microwell plates or test tubes. Our ABTS solution allows HRP reaction done in a single addition. The assay solution changes its color to light green upon its reaction with HRP or HRP conjugates in the presence of hydrogen peroxide.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
关键词：ReadiUse™ ABTS 底物溶液 *针对 HRP 偶联物的 ELISA 检测进行了优化*30931-67-0详细如下：
Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product. This chromogenic reaction is widely used for quantify HRP in ELISA assays. The oxidized ABTS product has the absorption maximum of 420 nm that can easily be followed with a spectrophotometer. ReadiUse™ ABTS Solution is optimized for ELISA assays that use HRP or HRP-labeled conjugates and hydrogen peroxide in microwell plates or test tubes. Our ABTS solution allows HRP reaction done in a single addition. The assay solution changes its color to light green upon its reaction with HRP or HRP conjugates in the presence of hydrogen peroxide.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-1120 | ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1123 | ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1130 | ReadiLink™ Rapid mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1131 | ReadiLink™ Rapid mFluor™ Red 780 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1150 | mFluor™ Violet 450 SE | 1mg |
| AAT-1151 | mFluor™ Violet 510 SE | 1mg |
| AAT-1152 | mFluor™ Violet 540 SE | 1mg |
| AAT-1160 | mFluor™ Blue 570 SE | 1mg |
| AAT-1165 | mFluor™ Green 620 SE | 1mg |
| AAT-1190 | mFluor™ Red 700 SE | 1mg |
| AAT-1191 | mFluor™ Red 780 SE | 1mg |
| AAT-1210 | mFluor™ Red 780 amine | 1mg |
| AAT-1220 | ReadiLink™ Rapid iFluor™ 350 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1227 | ReadiLink™ Rapid iFluor™ 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1230 | ReadiLink™ Rapid iFluor™ 594 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1235 | ReadiLink™ Rapid iFluor™ 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1240 | ReadiLink™ Rapid iFluor™ 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1245 | ReadiLink™ Rapid iFluor™ 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1250 | ReadiLink™ Rapid iFluor™ 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1255 | ReadiLink™ Rapid iFluor™ 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1260 | ReadiLink™ Rapid iFluor™ 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1265 | ReadiLink™ Rapid iFluor™ 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1299 | ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction* | 2Labelings |
| AAT-1300 | ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction* | 2Labelings |
| AAT-1310 | Buccutite™ Rapid PE Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction* | 2Labelings |
| AAT-1311 | Buccutite™ Rapid APC Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction* | 2Labelings |
| AAT-1360 | iFluor™ 790 acid | 5mg |
| AAT-1362 | iFluor™ 790 amine | 1mg |
| AAT-1364 | iFluor™ 790 hydrazide | 1mg |
| AAT-1366 | iFluor™ 790 maleimide | 1mg |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
理想的底物。H2O2的摩尔消光系数为10mm光径240nm波长下为43.6，因此，可通过这种方式来检测H2O2工作溶液的浓度。 固相ELISA中，当温度高于20oC时，HRP活性常较低，在底物溶液中加入非离子去垢剂聚山梨醇-20或TritonX-100可延迟HRP的失活，且可使反应温度加大，但非离子去垢剂的这种酶活性保护效应依氢供体的不同而有差异。如以2,2’-联氮-双-[3-乙基苯并噻唑啉]-6-磺酸(ABTS)为氢供体，则只能保护20%的酶活性，而以邻联茴香胺(ODA)为氢供体时，酶活
4倍。吸附于固相的HRP较游离的HRP更易受过量H2O2的抑制。如果30％H2O2贮存液浓度经测定证实确为30％，则稀释10 000—12 000倍常是较为理想的底物。H2O2的摩尔消光系数为10mm光径240nm波长下为43.6，因此，可通过这种方式来检测H2O2工作溶液的浓度。固相ELISA中，当温度高于20oC时，HRP活性常较低，在底物溶液中加入非离子去垢剂聚山梨醇-20或TritonX-100可延迟HRP的失活，且可使反应温度加大，但非离子去垢剂的这种酶活性保护效应依氢供体的不同而有
substrate into a colored solution. 阶段6：底物显色 Promptly washed the plate after incubation and add 100ul of ABTS substrate solution to each well. 孵育结束后，立即洗板。每孔加入100ul ABTS底物溶液 Use an Avidin-HRP in conjunction with ABTS only.
技术资料暂无技术资料 索取技术资料
ReadiUse™ ABTS 底物溶液 *针对 HRP 偶联物的 ELISA 检测进行了优化*30931-67-0
询价
