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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
PIK-90
- 库存:
咨询
- 供应商:
无锡云萃生物
- CAS号:
677338-12-4
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验5.99 5.82 5.69 5.60 5.52 5.46 5.37 5.27 5.17 5.06 4.96 4.85 6 7 6.54 5.89 5.52 5.28 5.12 4.99 4.90 4.82 4.76 4.67 4.57 4.47 4.36 4.25 4.14 7 8 6.06 5.42 5.05 4.82 4.65 4.53 4.43 4.36 4.29 4.20 4.10 4.00 3.89 3.78 3.67 8 9 5.71 5.08 4.72 4.48
" to determine proper settings. The standard we use, to generate predominately 2-5kb fragments is: Cycles: 20 Speed code:10-12.To make sure BAC DNA is in solution, incubate DNA at 37℃ for 30 minutes, vortexing hard every 10 min. Centrifuge at 12,000 rpm
Replication timing by density transfer
in Ycomp+[12 C]-glucose+ alpha factor. [I don't particularly try to resuspend the cells in exactly the same volume they were in originally, but rather, in a volume that's convenient.] 4. Transfer to 37o C, wait for culture to reach 37o C











