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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Mono-mehtyl-Histone H3(Lys4)
- 应用范围:
WB, ICC, IHC, FC
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat
- 保质期:
12个月
- 库存:
88
- 供应商:
上海博尔森生物科技有限公司
- 克隆性:
Rabbit polyclonal Antibody
- 保存条件:
详见说明书
- 形态:
详见说明
- 亚型:
IgG
- 免疫原:
peptide
- 规格:
50ul/100ul
| 规格: | 50ul | 产品价格: | ¥1200.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2100.0 |
抗体的概念:
抗体的结构图:
抗体的分类:
1.从制备方式的角度,分为多抗和单抗:
2.从实验应用的角度,分为一抗和二抗。
单抗和多抗的区别:
| 多克隆抗体 |
单克隆抗体 |
| 制备成本低廉 |
制备成本高昂 |
| 制备技能要求不高 |
应用技术之前需接受专门培训 |
| 制备速度较快 |
需要较长的时间制备杂交瘤细胞 |
| 产生大量非特异性抗体 |
产生大量特异性抗体 |
| 识别任一抗原上的多个表位 |
仅识别一个抗原上的单一表位 |
| 批次间可能存在差异 |
制得的杂交瘤细胞为持续且可再生产的单克隆抗体来源 |
| - |
批次间无差异或差异较小 |
一抗和二抗的关系:
抗体的应用:
抗体的用途:
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文献和实验Determination of Histone Methylation in Mono- and Dicotyledonous Plants
. It has been found that the methylation pattern in the histone H3 may provide understanding of the process involved in cell differentiation, adaptation, and evolution in plants. In this work, we detail a method for isolation of nuclear proteins from small amount of sample to identify
Site-directed hydroxy radical mapping of nucleosome positions in vitro
and Davey, 2003). Procedure Preparation of Derivatised Octamer Refold and purify histone octamer containing histones H2A wild type, H2B wild type, H3 Cys110Ala and H4 Ser47Cys following standard methods (Dyer et al., 2004) (see note 1);
Isolation of Histones and Nucleosome Cores from Mammalian Cells
is often important for understanding mechanisms of regulation of transcription and other processes that occur on DNA. The basic unit of chromatin is the nucleosome core, containing two copies each of the core histones H2A, H2B, H3, and H4 to form a histone octamer
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