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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Ubiquitin-like modifier-activating enzyme 1
- 应用范围:
WB, ICC/IF, IHC, FC
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 保质期:
12个月
- 库存:
88
- 供应商:
上海博尔森生物科技有限公司
- 克隆性:
Recombinant Rabbit monoclonal Antibody
- 保存条件:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 形态:
详见说明
- 亚型:
IgG
- 免疫原:
recombinant protein
- 规格:
50ul/100ul
| 规格: | 50ul | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2500.0 |
抗体的概念:
抗体的结构图:
抗体的分类:
1.从制备方式的角度,分为多抗和单抗:
2.从实验应用的角度,分为一抗和二抗。
单抗和多抗的区别:
| 多克隆抗体 |
单克隆抗体 |
| 制备成本低廉 |
制备成本高昂 |
| 制备技能要求不高 |
应用技术之前需接受专门培训 |
| 制备速度较快 |
需要较长的时间制备杂交瘤细胞 |
| 产生大量非特异性抗体 |
产生大量特异性抗体 |
| 识别任一抗原上的多个表位 |
仅识别一个抗原上的单一表位 |
| 批次间可能存在差异 |
制得的杂交瘤细胞为持续且可再生产的单克隆抗体来源 |
| - |
批次间无差异或差异较小 |
一抗和二抗的关系:
抗体的应用:
抗体的用途:
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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
Assays for RING Family Ubiquitin Ligases
through an enzymatic cascade of three discrete steps, which results in covalent attachment of ubiquitin to the substrate. The first two steps in this cascade involve the activating and conjugating enzymes, E1 and E2. The third and final step is performed by the E3
CDK-Activating Kinases: Detection and Activity Measurements
All cyclin-dependent kinases (CDKs) involved in eukaryotic cell cycle control require phosphorylation at a conserved threonine (or serine) residue within the activation- or T-loop to attain full enzymatic activity. The enzyme responsible
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