Dialyzed Fetal Bovine Serum

Primary Cultures of Astrocytes from Fetal Bovine Brain

differentiation and the prevalence of glial cells vs. neurons. We compare explants from early, middle, and late stages of development and two different fetal calf serum concentrations (1 and 10%) to identify the best conditions to obtain

Isolation of Microtubules (Bovine Brain)

minutes at 4° C for further clarification.   Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard

Culturing Fetal Precursor Cells Using Free Floating Serum-Free Conditions

can be expanded in vitro using both adherent and non-adherent culture protocols. The culture of fetal human neural precursors in the absence of serum as free-floating clusters of cells termed neurospheres is described here.