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文献和实验once with Buffer 1 by centrifugation at 400 x g for 8 minutes at 2–8°C. 8) Wash MNC twice with Buffer 1 by centrifugation at 225 x g for 8 minutes at 2–8°C and resuspend the MNC at 1 x 108 MNC per ml in Buffer 1. 3. Enrichment of DCs
through. 4) Repeat the wash step once. 5) Discard the tube and place the column into a clean Wash Tube, supplied with the kit. 6) Centrifuge the column at ≥12,000 × g for 2–3 minutes at room temperature to remove any residual Wash Buffer
Studies of the Ubiquitin Proteasome System
can, in general, be covalently modified by polypeptides, resulting in alterations in their fate and function. The first?identified and most well studied of these modifying polypeptides is ubiquitin. Although targeting for proteasomal degradation is the best
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