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文献和实验HIGH RESOLUTION GENETIC FOOTPRINTING
BY PCR FROM INSERT See note [9] in appendix. MATERIALS T4 polynucleotide kinase (New England Biolabs) Sephadex G-15 resin (swell in TE buffer), glass wool, 1 ml syringes (for making spin columns) Gene-specific primer(s). We typically
In Situ Proximity Ligation Assay for Microscopy and Flow Cytometry
) Aniline Desalting device (e.g., Zeba Desalt Spin Columns by Thermo Scientific, or illustra MicroSpin G‐50 by GE Healthcare
Determination of Protein Contacts by Chemical Cross‐Linking With EDC and Mass Spectrometry
or methanol Dialysis membrane or micro dialysis cups of appropriate MWCO (Pierce) or gel filtration spin column (MacroSpin columns; http://nestgrp.com, no. SMM
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