RED Device Insert Removal Tool

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

that is modified. Upon enzymatic digestion, in vitro or in Escherichia coli , double-strand breaks are generated leading to recombination between the vector and the insert. The procedure described here is thus an additional powerful tool for manipulating large

In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue

  DNA electrotransfer to muscle tissue yields long-term, high levels of gene expression; showing great promise for future gene therapy. We want to characterize the novel far-red fluorescent protein Katushka as a marker for gene

Embryo Dissection and Micromanipulation Tools

, such as the Dumont 5 or 5A (Fine Science Tools), are adequate for membrane removal and most micromanipulations. They do not rust easily, but they cannot be sharpened to as fine a point as carbon-steel forceps. To sharpen the forcep points, use a fine stone