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文献和实验Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads
during the amplification reaction. The DNA can then be immobilized onto streptavidin-coated paramagnetic beads, simultaneously removing buffers, dNTPs, and unincorporated PCR primers. By alkali denaturation, the immobilized double-stranded DNA is converted into a single
cDNA Library Construction Using Streptavidin-Paramagnetic Beads and PCR
cDNA clones of genes expressed in small amounts of material can be hard to obtain because the construction of conventional cDNA libraries requires microgram amounts of poly (A)+ RNA (1 ). The polymerase chain reaction (PCR), which is commonly
Mouse B cell isolation with magnetic beads
and incubate on ice for 15min; l Negative : 1.Resuspend cells with MACS to 400 ul and 80 ul B cell Biotin each spleen,incubate on ice for 15 min; 2. Add 300 MACS and 200 anti-biotin Beads each spleen, incubate on ice for 15 min.
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