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文献和实验Identification and Quantitation of Signal Molecule-Dependent Protein Phosphorylation
in combination with phosphopeptide enrichment by titanium dioxide (TiO2 ) and their identification by MS is described. This workflow can be used to gain insights into the role of signalling molecules such as cyclic nucleotides on regulatory networks
Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells
dioxide (TiO2 ) for phosphopeptide enrichment, high-resolution MS for peptide and protein identification, and stable isotope labeling by amino acids in cell culture (SILAC) for quantification. This approach allows us to identify thousands
Determining In Vivo Phosphorylation Sites Using Mass Spectrometry
Molecule) Support Protocol 1: Phosphopeptide Enrichment Using Protea Biosciences TiO2 Spin Tips Support Protocol 2: Phosphopeptide Enrichment Using the Phos‐Trap TiO2 Phosphopeptide Enrichment Kit
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