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文献和实验Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
) 10‐ml Sephadex G25 gel filtration column (essentially any standard desalting column can be used) Amicon Ultra centrifugal concentrators (MWCO, 10,000; Millipore, cat
, for a discussion of parameters affecting MWCO. Knowledge of the precise MWCO is usually not required; however, it is necessary only to use a membrane with a pore size that is much smaller than the macromolecule of interest. For most plasmid and protein dialyses
Imaging Protein‐Protein Interactions by Fluorescence Resonance Energy Transfer (FRET) Microscopy
) 1‐ml Protein G HiTrap columns (Amersham Pharmacia Biotech) Centricon YM30 concentrators (Amicon)
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