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文献和实验Automated Generic Analysis Tools for Protein Quantitation Using Stable Isotope Labeling
Isotope labeling combined with LC-MS/MS provides a robust platform for quantitative proteomics. Protein quantitation based on mass spectral data falls into two categories: one determined by MS/MS scans, e.g., iTRAQ-labeling quantitation
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
, and the characterization of protein interactions. Stable isotope labeling techniques have facilitated the precise quantitation of changes in protein abundance by mass spectrometry. Among different choices, Stable Isotope Labeling by Amino acids in Cell culture (SILAC
Protein Profiling of the Brain: Proteomics of Isolated Tissues and Cells
of protein turnover in both dividing and nondividing cells, a technology now known as pulse SILAC (pSILAC). The ability to profile proteomes of isolated cells or tissues of the brain opens new experimental opportunities in neuroscience
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