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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
), containing 1.2, 1.0, 0.8, and 0.6 M NaCl 6000 to 8000 MWCO dialysis tubing (Spectrapor; boil in Milli‐Q water for 5 min and store at 4°C
as well as the cumbersome task of clamping the dialysis tubing. Other vendors provide vessels for dialyzing multiple samples simultaneously. Selection of the best dialysis approach to be used will depend on a number of factors including the volumes to be dialyzed, the MWCO
Isolation of Histones and Nucleosome Cores from Mammalian Cells
., 21‐G needle infusion set with 12‐in. (30.5‐cm) tubing (Abbot Laboratories) 50°C water bath 6 to 8 kDa MWCO dialysis bag
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