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文献和实验Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
crude sonicated sample Dispense into 1.5 ml eppendorf tubes,spin 14k rpm,4℃,30 min Combine soluble fractions into 1 tube Take 30μl soluble fraction as post-sonication soluble sample Test solubility of target protein by SDS-PAGE/Coomassie Blue Stain
of DNA and RNA can be achieved. The purified DNA and RNA are eluted separately and ready to use in any downstream application. The kit is also available in spin-column format for micro- and mini-sized samples. Principle and procedure
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
ethanol (room temperature, 96-100%). Gently mix by inverting tube 6-7 times and incubate at room temperature for1-2 minutes. Plasmid DNA Purification with the HiBind® DNA Mini Column II: 8. Equilibrate the HiBind® DNA column: Add 100ul
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