HisPur Ni-NTA Spin Columns, 3m

Rapid screening of small expression cultures

Materials Ni_NTA Spin Columns LB medium Kanamycin stock solution Ampicillin stock solution IPTG stock solution Buffers A–D 5× SDS -PAGE sample buffer 1. Pick

Small scale His-Tag fusion protein purification under denaturative conditions

ml suspension (it means 500ml original culture). 5)    Spin 10min 8000rpm 4ºC, discharge supernatant 6)    Keep cell pellet at -80ºC   Equilibration of Ni-NTA agarose Place 50ul beads (100ul suspension) of Ni-NTA agarose beads in 1.5ml

Natural History Museum Protocol for EST sequencing from lamb

PCR reaction (32µl) into a 1.5ml microtube, vortex briefly to mix and then add 1ml of "Wizard Resin" and vortex briefly, 3 times over a 1 minute period remove the plunger from a 3ml, luer l℃k syringe and attach the barrel to a clean "Wizard