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文献和实验Fix gel in 50% methanol for greater than 1 hour. rinse gel in dH2O 3 times 5 minutes each place back in 50% methanol for 1 hour. Stain gel in 100 mls of stain solution for 30 minutes Rinse gel in dH2O 3 times 5 minutes each. Place gel
1.After electrophoresis of protein gel,transfer gel to round staining tray.Add ~200 ml protein gel stain. 2.Microwave for ~45 sec until the solution just starts to boil.Incubate at room temp with gentle shaking for 10-15 min.Heating allows the gel
SILVER STAIN OF PROTEIN GELS(Ⅱ)
1.Fix gel in 50% methanol for greater than 1 hour rinse gel in dH2O 3 times 5 minutes each place back in 50% methanol for 1 hour. 2.Stain gel in 100 mls of stain solution for 30 minutes 3.Rinse gel in dH2O 3 times 5 minutes each. 4.Place gel
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