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文献和实验Reducing Sample Complexity in Proteomicsby Chromatofocusing with Simple Buffer Mixtures
Chromatofocusing has many potential applications in the field of proteomics, such as for the isolation and removal of major sample components to facilitate the analysis of low-abundance components, and for sample prefractionation
amplification on 1-2% TBE agarose gel, as two or more replicate lanes. 2. Cut off 1 lane - flanked by marker DNA if desired, and notched to allow re-orientation with remainder of gel - and stain in preferred ethidium bromide concentration (I use 50 ng/ml for 10
Sample Solublization Buffers for Two-Dimensional Electrophoresis
Before two-dimensional electrophoresis (2-DE), proteins of the sample must be denatured, reduced, disaggregated, and solubilized. Sample solubilization is usually carried out in a buffer containing chaotropes (typically 9.5 M urea, or 5–8 M
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