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文献和实验Conjugation of Antibodies to Horseradish Peroxidase
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups
detected by an antifluorescein alkaline phosphatase conjugate, which in turn catalyzes highly sensitive chemiluminescent detection, is described in Chapter 8 .
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
after Peroxidase Quenching. 2) Add the digestion enzyme of choice to your tissue sections Ficin and pepsin may be applied directly from the bottle. Trypsin requires dilution: 1 drop Trypsin concentrate to 3 drops Trypsin Diluent. Mix well and apply
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