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文献和实验Blocking of unwanted non-specific staining
, which should be present in the development buffer. Blocking of endogenous fluorochromes. Blocking of endogenous fluorochromes is impossible. One may choose fluorochromes emitting in the UV range of spectrum, where endogenous autofluorescence of tissue is minimal.
Bromodeoxyuridine Staining of Mammary Tissue
from a post-pubertal mouse.) II. BrdU Detection A. Perfusion fixed, paraffin sections: 1. Deparaffinize sections using standard xylene/alcohol procedures. 2. Treat sections with 0.1% Trypsin in 0.1M TBS (pH7.5) + 0.1% CaCl2 for 20 minutes at 37
Bromodeoxyuridine Staining of Mammary Tissue
. BrdU Detection A. Perfusion fixed, paraffin sections: 1. Deparaffinize sections using standard xylene/alcohol procedures. 2. Treat sections with 0.1% Trypsin in 0.1M TBS (pH7.5) + 0.1% CaCl2 for 20 minutes at 37oC in a humid box. 3. Rinse 2 x
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