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文献和实验well in terms of background intensity, this is only a first, but definitely not a sufficient step in the selection of the surface blocker during professional assay development. A much more discriminating and important test is the determination of the coefficient
Western blotting using ECL reagent
REAGENTSECL Western blotting kit (Amersham Life Science; cat# RPN2108): contains second antibodies for both mouse and rabbit, substrate and milk blocker (the milk blocker is not normally used when using ruminant samples).Hybond ECL nitrocellulose
WESTERN BLOTTING USING CHEMILUMINESCENCE
REAGENTS ECL Western blotting kit (Amersham Life Science; cat# RPN2108): contains second antibodies for both mouse and rabbit, substrate and milk blocker (the milk blocker is not normally used when using ruminant samples). Hybond ECL
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