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文献和实验CCMB buffer to make chemical competent cell
buffer 5 Measurement of competence 6 5x Ligation Adjustment Buffer 7 References 1.Preparing glassware and media Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free
DIRECT ELISA USING FLUORESCENT SUBSTRATE PROTOCOL
: TBS 250 μl/well; 3x 30 seconds.8. Amplify signal by adding 100 μl/well AttoPhos Fluorescent substrate system, for 5-10 min at RT. 36 mg ofAttoPhos substrate should be mixed with 60 ml of AttoPhos buffer 24 hours prior to use. Make sure well isclean
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
58 to 0.1%.Store dilution buffer at -70 degrees.Proteins are diluted in dilution buffer and quick frozen on dry ice.Thaw proteins on ice.Proteins are typically stable to multiple repeated freeze thaw.5x binding buffer 1 ml20% glycerol 400 microliters
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