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文献和实验Antibody Precipitation with Protein A or G
#20333) or ImmunoPure® Immobilized Protein G (Pierce product #20398). Primary antibody Immunoprecipitation buffer: 20 mM sodium phosphate, pH 7.5, 500 mM NaCl, 0.1% SDS, 1% NP40, 0.5% sodium deoxycholate and 0.02% sodium
). Primary antibody Horseradish peroxidase-conjugated secondary antibody Wash Solution II (for 1L: 1 ml Tween-20, 100 ml 10X PBS, 900 ml distilled water) Luminol and oxidizing reagents (e.g., Pierce or NEN) 2. Methods
Immunogold Probes in Light Microscopy
All immunocytochemical techniques are based on the same principle of incubating the target antigen with an appropriate antibody solution, but they may incorporate one from a range of different microscopically dense markers for visualizing
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