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文献和实验Cloning by Limiting Dilution of Hybridoma
Author: Nanci Donacki Source: Contributed by Nanci Donacki Date Added: Tue May 14 2002 Date Modified: Tue Apr 27 2004 Materials DMEM
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
the medium and resuspend the cells in Chemotaxis Buffer at 0.5 to 1 X 106 cells/ml. Maintain cells at 37°C until the chemotaxis chambers are ready to use. C. Preparation of Agonist Solution(s) 1. Dilute the agonists (chemokines or other chemoattractants
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
实验试剂 Dulbecco’s Modified Eagle Medium (D-MEM) Fetal Bovine Serum (FBS), ESC-Qualified D-MEM/F-12 (1X) with GlutaMAX™-I KnockOut™ Serum Replacement (KSR) MEM Non-Essential Amino Acids Solution, 10 mM
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