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文献和实验Enrichment of Membrane Proteins by Partitioning in Detergent/Polymer Aqueous Two-Phase Systems
mitochondrial membrane proteins followed by enrichment of hydrophobic proteins in aqueous two-phase system. Combining the zwitterionic detergent Zwittergent 3–10 and the nonionic detergent Triton X-114 results in a complementary solubilization of proteins
Detergent-Extracted Models for the Study of Cilia or Flagella
in inducing motion but not coordinated swimming. Applying the glycerination technique to sea urchin sperm, Brokaw and Benedict (4 ) successfully restored coordinated beating of the flagellum and progressive swimming. Using the nonionic detergent Triton X-100
Zwittergent 3-10 and the nonionic detergent Triton X-114 results in a complementary solubilization of proteins, which is similar to that of the anionic detergent sodium dodecyl sulfate (SDS) but with the important advantage of being nondenaturing. Detergent
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