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文献和实验Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
Materials Polyacrylamide pre-cast gel SimplyBlue™ Coomassie protein stain, 1x pre-mixed solution (Coomassie blue dye binds to proteins (arginine, the aromatic amino acids, and histidine) allowing protein bands to be visualized
amount. Alternatively,the microwave step can be omitted and the gel destained an additional hour or overnight. -------------------------------------------------------------------------------- Solutions: Protein gel stain Add to a 500 ml bottle: 1.2 g Comassie Blue
Coomasie Blue Staining of Protein Gels
LEVEL I Materials Protein gel from Exercise 4.2 0.25% (w/v) Coomasie Brilliant Blue R 250 in methanol-water-glacial acetic acid (5-5-1), filtered immediately before use. 7% (v/v) acetic acid Commercial
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