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文献和实验PEG Preparation of Plasmid DNA
above (5). 10) Wash the once briefly in 70% ethanol and air dry for 5 minutes. 11) Re-dissolve in water or TE (10ul per ml of original bacterial culture), add an equal volume of 13% PEG 8000, 800mM NaCl, mix and incubate on ice for 30 minutes.
as described above (5). 10. Wash the once briefly in 70% ethanol and air dry for 5 minutes. 11. Re-dissolve in water or TE (10ul per ml of original bacterial culture), add an equal volume of 13% PEG 8000, 800mM NaCl, mix and incubate on ice for 30 minutes
(8-10 mm wide). DO NOT add EtBr to the agarose gel (otherwise this will mutate your DNA samples). 3. Add 10 μl of 6X agarose gel loading buffer to the digests, and load 30 μl of the digests into a lane in the agarose gel. Load 7 μl of 1 kb DNA ladder
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