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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
DMF Hydroxylamine hydrochloride NaOH Pellets PD10 Column or Sephadex G25 Sulfo-SMCC
in the termination mixes. Two separate termination mixes provided optimal overlap for sequence data starting in the polylinker and extending to approximately 600 bases from the priming site. This two-step format eliminated the need for the chase required in the Bst
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