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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
A Protein G Human IgG1 ++++ ++++ Human IgG2 ++++ ++++ Human IgG3 - ++++ Human IgG4 ++++ ++++ Mouse IgG1 + ++++ Mouse IgG
Purification of Immunoglobulin G (IgG)
may consist of purification of total IgG or subpopulations (e.g., subclasses) of IgG from antisera/ascitic fluid/culture supernatant, or the isolation of a particular antigen binding fraction from such fluids. The former can be achieved by biochemical
Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose
Affinity chromatography relies on the reversible interaction between a protein and a specific ligand immobilized in a chromatographic matrix. The sample is applied under conditions that favor specific binding to the ligand as the result
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