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文献和实验the sample before IEF, particulates can cause smearing and may block gel pores. This technique is also very useful for removing unwanted components such as nucleic acids and some polysaccharides. :: SD buffer components. The components of the sample
of each component. 2D gel electrophoresis is generally used as a component of proteomics and is the step used for the isolation of proteins for further characterisation by mass spectroscopy. In the lab this technique is used for 2 main purposes, firstly
SDS Gel Electrophoresis of Tubulin\MAPs
Materials Stock Acrylamide: (30%T:0.8%C) 30% by weight of acrylamide 0.8% by weight of N,N'-bis-methylene acrylamide Separation Gel (Final Concentrations) 10% acrylamide
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