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文献和实验PCR product purification-Molecular Biology
product on agarose gel 1% check you DNA band.If your DNA band is too low, pool 2 or 3 PCR and run on agarose gel for purification.Use the minimum agarose% to minimise lost of sample.I'm using Qiagen minielute and it give me great results in a range of 400
how to purify small fragment of DNA-Molecular Biology
separation. You can use NuSieve GTG agarose (FMC Bioproducts). It is for separating fragments that are under 1 kb in length. You then cut out the band, and the agarose can be digested with beta-agarase. -Cthulhu- ----
Cloning long fragments-Molecular Biology
! But all the cloning I was doing the same way afterwards,was successful! I personally think molecular biology is kind of Voodoo.You never know,what gonna happen!So,the only thing I changed,was the ligation time.I think,it worth to try.Good luckClaude-claude----
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