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文献和实验Dual- and Triple-Color Fluorospot
in sterile filtered (0.2 μm) PBS (Mabtech). 实验设备 1. Transparent, flat-bottomed, 96-well plates with polyvinylidene difluoride (PVDF) membranes designed for low autofluorescence (IPFL; Millipore, Cork, Ireland). 2. Analysis
derived monoclonal antibodies, XH1 and XH2, to compete with the well-characterized ZO-1 anti-YFP1 antibody for binding to the target antigen YFP1; hypothetical antibody PC10 is used as a control. First, 96-well plates are coated with the YFP1 antigen
Materials: Capillary tubes 1.5 mL Eppendorf microfuge tubes 15 mL conical centrifuge tubes 96-well V-bottom plates (Corning Costar 3894, from Fisher) Flow tubes (Falcon 352054) Reagents: 0.5 M EDTA pH 8.0 PBS + 0.5% heat
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