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文献和实验Whole mount antibody staining of zebrafish embryos for markers of segmentation
6. Wash for a minimum of 2 hours with several changes of 0.2% saponin in PBS (can store in cold room at this point). 7. Wash with 1 ml HRP substrate buffer. 8. Develop with HRP substrate (metal-enhanced DAB). Monitor under dissecting microscope
for primary antibody), labeled with alkaline phosphatase or horseradish peroxidase. Substrate appropriate to the enzyme conjugate (HRP or AP). Appropriate wash buffer: Phosphate-buffered saline (PBST): 10 mM sodium phosphate, pH 7.2, 0.9% (w/v
secondary antibody review -- data from 99 publications
Alexa Fluor 596 immunocytochemistry 1:200 detect Smad2 in human 293T cells Invitrogen 13 Alexa Fluor 488 immunocytochemistry 13 IgG HRP western blot 1:3000 detect expression of CR-1 in 293T cells Amersham Biosciences 14
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