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文献和实验Preparation of Broth and Plates, etc.
4 (make 1.85 gm KH2PO4 and 10 gm K2HPO4 up to 80 ml in ddH2O; autoclave) and autoclave. 4) LB Plates Make 25.5 gm of LB Agar (Gibco #M27000C) up to 800 ml in ddH2O. Swirl, then add 110 µl of 10 N NaOH. Autoclave
Mag-Bind Plant RNA Protocol using 96-well Plate
rotor for 96 well plates 2. Sealing Film 3. 2 ml deep-well plate (Cat#EZ9602-02 ) 4. 500 μl round well processing plate (Cat#EZ9604-02 ) 5. Equipment for disrupting plant tissue (MM300 Mixer Mill or Geno/Grinder 2000)
DNeasy 96植物技术:使用搅拌磨MM 300从新鲜植物叶片中分离DNA
可再收集利用)。Do not transfer more than 400 µl of the supernatant otherwise the capacity of the DNeasy 96 Plates and the S-Blocks used in subsequent steps will be exceeded(转移的上清不要超过400 µl,否则在以后的步骤中将超出DNeasy 96 Plates和S-Blocks的负载量)。If less than 400 µl
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