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文献和实验Quantitation of Protein:Folin - phenol Reagent Method (Lowry Method)
- phenol reagent B : Add l00g of Na 2 WO 4 • 2H 2 O, 25g of Na 2 MoO 4 • 2H 2 O, 700ml of distilled water, 50ml of 85% H 3 PO 4 and 100ml of thick HC1 into a 1.5L round- bottom stoppered flask, mix
, sample, and reagent and add to the side of the wells. Use separate troughs/reservoirs for each reagent.——做ELISA时,加试剂不是应该直接加到底部的吗?怎么我看这里写的好像是要加到壁上。 2、Perform a time course using various kinase concentrations, including a no-enzyme blank, to confirm
Quantitation of Protein:Bicinchoninic Acid (BCA) Method
(2%),1.6g Na 2 C 4 H 4 O 6• 2H 2 O (0.16%),4g NaOH (0.4%) ,9.5g NaHCO 3 (0.95) respectively. Add water to 1L, and then adjust the pH to 11.25 by NaOH or solid NaHCO 3 ② Reagent B, 50ml: Take 2g
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