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文献和实验Vybrant® DyeCycle Green and Orange Stains
before staining Use 37?C for incubation with the Vybrant® DyeCycle™ stains, keep cells at 37?C until acquisition Staining may be performed at room temperature, with the staining time about twice as long as the 37?C incubation time
Dynabeads® Co-Immunoprecipitation Kit
, although the coupling reaction also works well with antibodies in storage buffers that include protein additives (e.g. BSA) and/or sodium azide (NaN3). This kit is not recommended for use with antibodies that have been stabilized in glycerol. 实验试剂
实验步骤 1. Measure out the amount of Taq DNA polymerase needed. When using a reaction cocktail, determine the total number of units in the mix. 2. Add an amount of “equivalent units” of Platinum® Taq
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