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- 详细信息
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- 文献和实验
- 技术资料
- 保质期:
见产品说明书
- 英文名:
Dispase II (neutral protease
- 库存:
大量
- 供应商:
罗氏
- 保存条件:
见产品说明书
- 规格:
5*1g
Dispase II (neutral protease), Dispase酶Ⅱ(中性蛋白酶)
4942078001,5 x 1 g non-sterile
EC 3.4.24.4
Enzyme activity: ≥0.8 U/mg (37°C, casein as substrate, pH 7.5)
Inhibitors: EDTA, EGTA, Hg 2+, other heavy metals. Dispase is not inhibited by serum.
Specificity: Dispase is a nonspecific metalloprotease.
Working concentration: 0.6 to 2.4 U/ml
Background Information
One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 minute under assay conditions. A practical comparison of Roche units of Dispase ® II with those cited in the Japanese literature (where dispase concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.
Contents
5 vials with 1 g each; non-sterile lyophilizate
Preparation of Stock and Working Solution:
Dissolve the non-sterile lyophilized enzyme in Hepes-buffered saline (50 mM Hepes/KOH pH 7.4, 150 mM NaCl) (10 mg/ml). Dilute further with the culture medium to be used for the isolated cells to a final concentration of 0.6 to 2.4 U/ml. Concentrations higher than 2.4 U/ml are not recommended. Sterilize through a 0.22 μm filter membrane.
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文献和实验®尤其在皮肤组织的表皮分离和上皮层细胞培养中获得了广泛的应用。Dispase®I与Dispase®II两者的差别在于纯度的不同,Dispase®I经过滤除菌,且每mg Dispase®I的纯度和酶活远高于Dispase®II。 罗氏高纯度研究级别酶混合物Liberase Research Grade Enzyme Blends Liberase Research Grade Enzyme Blends包含了一系列酶混合物,它们由不同比例的中性蛋白酶(非梭菌蛋白酶)和高纯
实验材料:1. 完整的人角膜;2. GMF-Saline G;3. 中性蛋白酶Ⅱ,1.2U/ml(用DMEM/F12/GASP稀释2.4U中性蛋白酶Ⅱ);4. 胰蛋白酶/EDTA;5. DMEM/F12/GASP;6. DEME/F12/2FB/GASP:DMEM/F12/GASP含2%FBS;7. CMF/GASP;8. LSC培养基;9. 弯虹膜剪,11cm(4-3/8in);10. Jeweler’s镊,10cm(4in);11. 角膜剪,19mm刃,尖头;12. Colibri缝纫钳
6. 分化培养基:为DMEM/F12按1:1(体积比)比例配制的混合培养基,添加5μg/ml胰岛素、1μg/ml氢化可的松、3μg/ml催乳素和50μg/ml庆大霉素。其中,催乳素以3μg/ml浓度溶于10mmol/L NaOH中,过滤除菌,4℃下可保存2周,使用前添加到培养基中。 7. C中性蛋白酶溶液:为9.6μg/ml的Dispase溶液,用DMEM配制。 8. 手术刀、解剖剪、解剖镊、眼科剪,眼科
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