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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Powder: -20°C, 3 years.In solvent: -80°C, 6 months; -20°C, 1 month.
- 库存:
货期:询盘
- 供应商:
MedChemExpress LLC
- CAS号:
168293-15-0
- 规格:
询盘
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3-Epidehydropachymic acid
CAS No. : 168293-15-0
MCE 国际站:3-Epidehydropachymic acid
产品活性:3-Epidehydropachymic acid,一种羊毛甾烷三萜,对人急性单核细胞白血病细胞系 THP-1 具有毒性作用 (IC50=52.51 μM)。
研究领域:Others
作用靶点:Others
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文献和实验Purpose Materials10ml 6% dextran + 7ml citrate/citric acidDextran: T500 --> 6g+100ml PBSCitrate solution: 25g Na Citrate + 8g citric acid + 500 ml PBS 43 ml blood 12 ml RT Histopaque 107718 ml cold H2 O2 ml 10x PBSM199 for HUVECs: 1L powder pocket
4.1 ), using 15% acrylamide (15%T:5%C) in 2.5 M urea and 0.9 M acetic acid. Set up the gel in the electrophoresis unit and run the gel at 2 mA/gel for 2 hours with no sample, using 0.9 M acetic acid for the running buffer. Dissolve
James Hardwick angiotensin assay protocol
that are removed at each time point are mixed immediately with 15 microliters of 5% trichloroacetic acid (TCA). Let the samples sit on ice for at least 15 min to precipitate proteins. 4. Spin the samples in a microcentrifuge for 5 min at 4°C.
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