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文献和实验Permeability Assessment Using 5-day Cultured Caco-2 Cell Monolayers
In this chapter, we have provided a step-by-step protocol of an accelerated differentiation 5 day culturing Caco-2 (HTB-37) model in a 24-well plate format. The 5-day accelerated Caco-2 assay is based on previous literature protocols
Use of Caco-2 Cell Monolayers to Study Drug Absorption and Metabolism
The Caco-2 cell culture model is used to determine the absorption potentials of drug candidates and the transport and metabolism mechanisms of drugs and dietary chemicals. The Food and Drug Administration (FDA) recognized the model
Acetaldehyde-induced Barrier Disruption and Paracellular Permeability in Caco-2 Cell Monolayer
. We showed that acetaldehyde disrupts intestinal epithelial tight junctions and increases paracellular permeability to endotoxins in Caco-2 cell monolayer, the extensively studied model of the differentiated intestinal epithelium. The mechanisms involved
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