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- YQ77120
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
咨询
- 保质期：
咨询
- 英文名：
4-Chloro-6-iodoquinoline-3-carbonitrile
- 库存：
咨询
- 供应商：
无锡云萃生物
- CAS号：
214483-20-2
- 规格：
2mg/10mg/500mg/500g
| 规格： | 2mg | 产品价格： | ￥100.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥100.0 |
| 规格： | 500mg | 产品价格： | ￥100.0 |
| 规格： | 500g | 产品价格： | ￥100.0 |
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
). However, for localization of cells containing transduced lacZ, chromogenic substrates that yield a precipitated product are desirable (Holt and O'Sullivan, 1958, Pearse 1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro
Methods for use with the mTn-lacZ/LEU2-mutagenized library
. Screening 30, 000 transformants should give you 95% coverage of the yeast genome. To minimize double integrants, transformations should contain the lowest amount of DNA practicable. We therefore recommend that a pilot experiment be performed
