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- 英文名：
Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Microplate Reader*
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
200Tests
Cell Meter™荧光细胞内一氧hua氮（NO）活性测定试剂盒详细如下
Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes. Altered NO production is implicated in various immunological, cardiovascular, neurodegenerative and inflammatory diseases. As a free radical, NO is rapidly oxidized and there is relatively low concentrations of NO existing in vivo. It has been challenging to detect and understand the role of NO in biological systems. Cell Meter™ Fluorimetric Intracellular Nitric Oxide Assay Kit provides a sensitive tool to monitor intracellular NO level in live cells. Nitrixyte™ probes are developed and used in our kit as an excellent replacement for DAF-2 for the detection and imaging of free NO in cells. Compared to the commonly used DAF-2 probe, Nitrixyte™ probes have better photostability and enhanced cell permeability. This particular kit uses Nitrixyte™ Orange that can react with NO to generate a bright orange fluorescent product that has spectral properties similar to Cy3® and TRITC. Nitrixyte™ Orange can be readily loaded into live cells, and its fluorescence signal can be conveniently monitored using the filter set of Cy3® or TRITC. This kit is optimized for fluorescence imaging and microplate reader applications.
Components
Component A: 500X Nitrixyte™ Orange 1 vial (50 µL)
Component B: Assay Buffer I 1 bottle (20 mL)
Component C: Assay Buffer II 1 bottle (20 mL)
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文献和实验〔l〕在中生动物二胚虫类的体表细胞中，体前端二环列并排的 8— 9个细胞称为极细胞。以其密生短纤毛和细胞体为小的多面体这一点，与其下面的体表细胞（躯细胞）相区别。第一环列的 4个细胞称为前极细胞（ propo-lar cell），而第二环列的 4— 5个细胞称为后极细胞（ metapolar cell）。这些细胞可附着在寄主（章鱼、乌贼）的肾囊壁上或用以穿孔肾囊壁游出外界。在躯干细胞前端有 2个类似极细胞的细胞，特称为侧极细胞（ parapolar cell）。〔 2〕在昆虫的胚胞形成前
characteristics, depending on the media, the temperature and the strain of cells utilized. For bacteria (and some algae, fungi and other eukaryotic tissue culture) it is possible to measure the growth of cell populations by calculating cell number or mass
因子，还有几个方面仍有待于表征，例如这些工具的相对优势、脱靶活性的确切水平以及体内传递这些工具的能力。但是，研究人员仍然预见到将有许多激动人心的应用，例如通过调节内源性位点上的表达，探测新类别的非编码基因功能，以及应用于人类基因治疗的新范式。 两份Cell子刊联合发布CRISPR专刊：七篇文章CRISPR的最新前沿⑦Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors
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