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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C, protect from light
- 英文名：
VDP-green
- 库存：
货期：1-2天
- 供应商：
MedChemExpress LLC
- CAS号：
2937705-58-1
- 规格：
1 mg/5 mg
| 规格： | 1 mg | 产品价格： | ￥2000.0 |
|---|---|---|---|
| 规格： | 5 mg | 产品价格： | ￥4900.0 |
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AC-green
CAS No. : 2937705-58-1
MCE 国际站：AC-green
产品活性：AC-green (VDP-green) 是一种 β-烯丙基氨基甲酸酯荧光探针，可选择性地对活体系统中的邻位二硫醇蛋白质 (VDPs) 进行成像 (λex/λem=400/475 nm)。AC-green 可以高灵敏度检测降低牛血清白蛋白 (rBSA)。AC-green 毒性低，灵敏度高，适用于检测活细胞和斑马鱼中的 VDP。
研究领域：Others
作用靶点：Fluorescent Dye
In Vitro: AC-green (VDP-green) can respond to VDPs with more than 60-fold increase of emission in aqueous solution, while there is no significant interference from biological thiols, amino acids or inorganic salts.
AC-green (2 μM; 90 min) has no apparent fluorescence signal within the pH range of 5.0-9.0. Addition of rBSA turns on the fluorescence.
AC-gree (10 μM) has low cytotoxicity in HepG2 cells and Hela cells.
AC-gree (10 μM; for 15 min) images VDPs in living HepG2 cells and bright green fluorescence appeared. This fluorescence is inhibited when the cells are pretreated with PAO, a popular specificligand for protein vicinal dithiols.
AC-gree (10 μM; for 20 min) incubates with zebrafishes has a strong fluorescence signal appeared in the green channel.
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文献和实验子序列等。引物的延伸是从 3' 端开始的，不能进行任何修饰。3' 端也不能有形成任何二级结构可能。 10、扩增产物的单链不能形成二级结构 某些引物无效的主要原因是扩增产物单链二级结构的影响，选择扩增片段时最好避开二级结构区域。用有关软件（比如RNAstructure）可以预测估计 mRNA 的稳定二级结构，有助于选择模板。实验表明，待扩区域自由能（△G°）小于58.6lkJ/mol时，扩增往往不能成功。若不能避开这一区域时，用7-deaza-2'-脱氧 GTP 取代 dGTP 对扩增的成功是有帮助
(and potential confusion) comes about with regard to SOLiD. There are 4 oligos for every dye, meaning there are four dinucleotides that are encoded by each dye. For example (see Fig.4), the dinucleotides CA, AC, TG, and GT are all encoded by the green dye
|998894|gb|S78179.1|S78179 Oryctolagus cuniculus type XII... 32 0.037 gi|31072033|gb|AC122153.4| Oryctolagus cuniculus clone LB1-... 28 0.58 gi|1496|emb|X52496.1|OCCAATP Rabbit mRNA for calcium ATPase 28 0.58 gi|1468|emb|X02814.1|OCATPDR Rabbit mRNA
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